The accumulation of formamide during the fermentation of histidine by Clostridium tetanomorphum.

نویسندگان

  • J T WACHSMAN
  • H A BARKER
چکیده

A histidine fermenting Clostridium was isolated in this laboratory by Dr. Arthur Kornberg by standard enrichment culture techniques. This bacterium, which can use either L-histidine or i-glutamic acid as a primary energy source, has been identified as Clostridium tetanomorphum (Breed et al., 1948). Cell suspensions of the organism have been shown to form carbon dioxide, ammonia, hydrogen, formate, acetate, and butyrate from L-histidine. The accumulation of at least one additional nitrogenous product is indicated by the relatively low (67-80 per cent) recovery of the nitrogen of histidine as ammonia. This paper demonstrates that formamide is a product of histidine fermentation and shows that it originates from the carbon at position 2 and the 5-nitrogen of the imidazole ring.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Formation of n-Butanol from d-Glucose by Strains of the "Clostridium tetanomorphum" Group.

A clostridial strain has been isolated that produced n-butanol, ethanol, butyrate, and acetate as major fermentation products from glucose but no acetone. At a pH of 6.6, n-butanol was formed by this microorganism only during growth. On the basis of its physiological characteristics and DNA-DNA homology data, the strain was assigned to the "Clostridium tetanomorphum" group (S. Nakamura, I. Okad...

متن کامل

Role of Butyryl Phosphate in the Energy Metabolism of Clostridium Tetanomorphum.

Twarog, R. (University of Illinois, Urbana) and R. S. Wolfe. Role of butyryl phosphate in the energy metabolism of Clostridium tetanomorphum. J. Bacteriol. 86:112-117. 1963.-A partially purified butyrokinase from Clostridium tetanomorphum has been found to phosphorylate valerate, butyrate, isobutyrate, and propionate. The divalent cation requirement is satisfied with magnesium. The pH optimum l...

متن کامل

The role of alpha-ketoglutarate and mesaconate in glutamate fermentation by Clostridium tetanomorphum.

The products of L-glutamate fermentation by resting or growing cells of Clostridium tetanomorphum are acetate, butyrate, hydrogen, and approximately stoichiometric amounts of carbon dioxide and ammonia (14). Experiments with C14-labeled glutamate have shown that acetate, butyrate, and carbon dioxide originate preferentially from glutamate carbons 1 and 2, 3 and 4, and 5, respectively, and that ...

متن کامل

Cloning and sequencing of glutamate mutase component E from Clostridium tetanomorphum. Organization of the mut genes.

The gene encoding component E, the large subunit, of adenosylcobalamin (coenzyme B12)-dependent glutamate mutase from Clostridium tetanomorphum has been cloned and sequenced. The mutE gene encodes a protein of 485 amino acid residues, with M(r) 53,708. The mutE gene is situated some 1,400 bp downstream of the mutS gene, which encodes the small subunit of glutamate mutase. Between the two is an ...

متن کامل

The Utilization of Amino Acids and Related Compounds by Clostridium tetani.

Studies on the metabolism of Clostridium tetani have been confined to the general changes during growth in various media. Woods and Clifton, 1937, and Barker, 1937, reported that Clostridium tetanomorphum, an anaerobe apparently closely related to C. tetani, obtains its energy by the fermentation of certain amino acids, while Clostridium sporogenes (Stickland, 1934-35) and C. botulinum (Clifton...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Journal of bacteriology

دوره 69 1  شماره 

صفحات  -

تاریخ انتشار 1955